VESTNIK
Bashkirskogo universiteta

RUSSIAN
ISSN 1998-4812

Archive | Volume 20, 2015, No. 3.

HIGH GENETIC POLYMORPHISM IN A POPULATION OF THE ENGLISH OAK IN THE LOWLANDS OF WESTERN URAL’S MACRO-SLOPE

Vestnik Bashkirskogo Universiteta. 2015. Vol. 20. No. 3. Pp. 854-856.
Gabitova A. A.
Bashkir State University; Bashkir State Agrarian University
32 Zaki Validi St., 450076 Ufa, Republic of Bashkortostan, Russia; 34 50-letiya Oktyabrya St., 450054 Ufa, Republic of Bashkortostan, Russia.
Email: abigabi@yandex.ru
Yanbaev R. Yu.
Bashkir State University; Bashkir State Agrarian University
32 Zaki Validi St., 450076 Ufa, Republic of Bashkortostan, Russia; 34 50-letiya Oktyabrya St., 450054 Ufa, Republic of Bashkortostan, Russia.
Redkina N. N.
Bashkir State University
32 Zaki Validi St., 450076 Ufa, Republic of Bashkortostan, Russia.

Abstract

A comparative study of polymorphism of populations of English oak within the South-Ural part of the area of distribution was carried out by using allozymes and ISSR analyses. Both methods showed that the genetic polymorphism is the largest level in the lowlands of western macro-slope of the mountains (Arkhangelsk forest district, the Republic of Bashkortostan). 25 alleles were found in isozyme loci Skdh-1, Fdh-1, Aap-1, Lap-2 and Dia-1 representing 92.6% of the total allelic diversity in oak forests of the area about 400x700 km. In this population, 55 polymorphic DNA fragments were detected (82.1% of the regional polymorphism) by using 5 ISSR primers. Relatively high genetic polymorphism can be explained by the history of oak stands in lowlands of the western Ural’s macro-slope. A gene pool of ancient neogene refugia oak populations after Pleistocene cold spells can be presented there. The genetic variability can be increased at times of spatial oak expansion from Volga refuges. The territory of the Arkhangelsk region, where the species is one of key components of broad-leaved forest zone, is adjacent to zone of broad-leaved-coniferous forests with subzone of the southern taiga, zone of boreal forests with subzone of pine and birch stands, zone of broad-leaved forests with subzone of deciduous stands, forest-steppe zone and steppes. Each of the zones includes English oak, which provides an opportunity to have powerful gene flow in territories of junction. It was shown that the used genetic markers are informative to select objects of regional genetic conservation in situ. The results also are useful to improve seed farming of the species and to increase efficiency of artificial reproduction of oak forests in the region.

Keywords

  • • English oak
  • • the Southern Urals
  • • polymorphism
  • • ISSR-analysis
  • • electrophoresis
  • • isozymes
  • • allozyme loci

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